Cdc25A Antibody
| Code | Size | Price |
|---|
| PSI-62-211-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Background:
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated.
Background References:
- Ito, Y., et al., Int. J. Mol. Med. 13(3):431-435 (2004).
- Nemoto, K., et al., Prostate 58(1):95-102 (2004).
- Goloudina, A., et al., Cell Cycle 2(5):473-478 (2003).
- Chen, M.S., et al., Mol. Cell. Biol. 23(21):7488-7497 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Cdc25A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-300 amino acids from the Central region of human Cdc25A.
NCBI Gene ID #:
993
NCBI Official Name:
M-phase inducer phosphatase 1
NCBI Official Symbol:
CDC25A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
59 kDa
Protein Accession #:
P30304
Protein GI Number:
50403734
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle
Swissprot #:
P30304
User NOte:
Optimal dilutions for each application to be determined by the researcher.