ALPPL2 Antibody

ProSci
Product Code: PSI-57-114
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-114-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A549 cell line lysates (35ug/lane).
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ALPPL2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in A549 cell line lysates (35ug/lane).
ALPPL2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Alkaline phosphatase, placental-like, ALP-1, Alkaline phosphatase Nagao isozyme, Germ cell alkaline phosphatase, GCAP, Placental alkaline phosphatase-like, PLAP-like, ALPPL2, ALPPL
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase.
Background References:
  • Pelttari, K., et al. Arthritis Rheum. 54(10):3254-3266(2006)
  • Nakano, T., et al. Biochem. Biophys. Res. Commun. 341(1):33-38(2006)
  • Hillier, L.W., et al. Nature 434(7034):724-731(2005)
  • Le Du, M.H., et al. J. Biol. Chem. 277(51):49808-49814(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ALPPL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the N-terminal region of human ALPPL2.
NCBI Gene ID #:
251
NCBI Official Name:
Alkaline phosphatase, placental-like
NCBI Official Symbol:
ALPPL2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa
Protein Accession #:
P10696
Protein GI Number:
145559564
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle
Swissprot #:
P10696
User NOte:
Optimal dilutions for each application to be determined by the researcher.