HAUS3 Antibody

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ProSci
Product Code: PSI-55-771
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-771-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
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HAUS3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
HAUS3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
HAUS augmin-like complex subunit 3, HAUS3, C4orf15
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
HAUS3 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).
Background References:
  • Shah, S.P., et al. Nature 461(7265):809-813(2009)
  • Lawo, S., et al. Curr. Biol. 19(10):816-826(2009)
  • Uehara, R., et al. Proc. Natl. Acad. Sci. U.S.A. 106(17):6998-7003(2009)
  • Goshima, G., et al. J. Cell Biol. 181(3):421-429(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HAUS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 372-400 amino acids from the Central region of human HAUS3.
NCBI Gene ID #:
79441
NCBI Official Name:
HAUS augmin-like complex subunit 3
NCBI Official Symbol:
HAUS3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
70 kDa
Protein Accession #:
Q68CZ6
Protein GI Number:
74708788
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle
Swissprot #:
Q68CZ6
User NOte:
Optimal dilutions for each application to be determined by the researcher.