SERPINA4 Antibody

ProSci
Product Code: PSI-62-924
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-924-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of SERPINA4 antibody in NCI-H460 cell line lysates (35ug/lane)
2 / 3
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SERPINA4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3

Western blot analysis of SERPINA4 antibody in NCI-H460 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SERPINA4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Kallistatin, Kallikrein inhibitor, Peptidase inhibitor 4, PI-4, Serpin A4, SERPINA4, KST, PI4
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
SERPINA4 inhibits human amidolytic and kininogenase activities of tissue kallikrein. The inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.
Background References:
  • Chai K.X., Chen L.-M.J. Biol. Chem. 268:24498-24505(1993)
  • Chai K.X., Ward D.C.Genomics 23:370-378(1994)
  • Zhou G.X., Chao L.J. Biol. Chem. 267:25873-25880(1992)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SERPINA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-320 amino acids from the C-terminal region of human SERPINA4.
NCBI Gene ID #:
5267
NCBI Official Name:
Kallistatin
NCBI Official Symbol:
SERPINA4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
Protein Accession #:
P29622
Protein GI Number:
68067608
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle
Swissprot #:
P29622
User NOte:
Optimal dilutions for each application to be determined by the researcher.