RB1 Antibody

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ProSci
Product Code: PSI-62-205
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-205-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Daudi,Raji cell line lysates (35ug/lane).This demonstratdetected the Rb protein (arrow).
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Confocal immunofluorescent analysis of RB1 Antibody (S608) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
3 / 3
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Western blot analysis in Daudi,Raji cell line lysates (35ug/lane).This demonstratdetected the Rb protein (arrow).
Confocal immunofluorescent analysis of RB1 Antibody (S608) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110, RB1
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50
Background:
The retinoblastoma protein is a tumor suppressor protein that is dysfunctional in many types of cancer.[1] One highly studied function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. pRb belongs to the pocket protein family, whose members have a pocket for the functional binding of other proteins.[2][3] Should an oncogenic protein, such as those produced by cells infected by high-risk types of human papillomaviruses, bind and inactivate pRb, this can lead to cancer. Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral.
Background References:
  • Dasgupta, P., et al., Mol. Cell. Biol. 24(21):9527-9541 (2004).
  • Cui, X., et al., Hum. Pathol. 35(10):1189-1195 (2004).
  • Borah, S., et al., J. Virol. 78(19):10336-10347 (2004).
  • Dasgupta, P., et al., J. Biol. Chem. 279(37):38762-38769 (2004).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-615 amino acids from human RB1.
NCBI Gene ID #:
5925
NCBI Official Name:
Retinoblastoma-associated protein
NCBI Official Symbol:
RB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
106 kDa
Protein Accession #:
P06400
Protein GI Number:
132164
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle
Swissprot #:
P06400
User NOte:
Optimal dilutions for each application to be determined by the researcher.