PCNA Antibody

Product Code: PSI-61-536

Supplier: ProSci

Code	Size	Price

PSI-61-536-400ul

400ul

£626.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: Rabbit Ig

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species: Human

Applications:

Immunofluorescence (IF)
Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Storage:

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of PCNA Antibody in Jurkat, Hela, 293 cell line lysates (35ug/lane)

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Western blot analysis of PCNA using rabbit polyclonal PCNA Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PCNA gene.

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Confocal immunofluorescent analysis of PCNA Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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Confocal immunofluorescent analysis of PCNA Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

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PCNA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

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Documents

Further Information

Additional Names:

Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA

Application Note:

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

Background:

PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.

Background References:

Wang,Y., J. Cell. Biochem. 106 (3), 409-413 (2009) Maga,G., Proc. Natl. Acad. Sci. U.S.A. 105 (52), 20689-20694 (2008) Acharya,N., Proc. Natl. Acad. Sci. U.S.A. 105 (46), 17724-17729 (2008)

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Homology:

Predicted species reactivity based on immunogen sequence: Bovine, Hamster, Monkey, Rat

Immunogen:

This PCNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-261 amino acids from the C-terminal region of human PCNA.

NCBI Gene ID #:

5111

NCBI Official Name:

Proliferating cell nuclear antigen

NCBI Official Symbol:

PCNA

NCBI Organism:

Homo sapiens

Physical State:

Liquid

PREDICTED MOLECULAR WEIGHT:

29 kDa

Protein Accession #:

P12004

Protein GI Number:

129694

Purification:

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Research Area:

Cancer,Cell Cycle

Swissprot #:

P12004

User NOte:

Optimal dilutions for each application to be determined by the researcher.