Alkaline Phosphatase Antibody

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ProSci
Product Code: PSI-58-008
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-58-008-400ul400ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysate from human placenta tissue lysate, using ALPI Antibody at 1:1000.
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Immunohistochemical analysis of paraffin-embedded H. small intestine section using Alkaline Phosphatase (ALPI) Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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4 / 4
Fluorescent confocal image of HeLa cells stained with Alkaline Phosphatase (ALPI) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with Alkaline Phosphatase (ALPI) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Alkaline Phosphatase (ALPI) immunoreactivity is localized to the cytoplasm of HeLa cells.
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Western blot analysis of lysate from human placenta tissue lysate, using ALPI Antibody at 1:1000.
Immunohistochemical analysis of paraffin-embedded H. small intestine section using Alkaline Phosphatase (ALPI) Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Fluorescent confocal image of HeLa cells stained with Alkaline Phosphatase (ALPI) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with Alkaline Phosphatase (ALPI) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Alkaline Phosphatase (ALPI) immunoreactivity is localized to the cytoplasm of HeLa cells.

Documents

Further Information

Additional Names:
Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, ALPI
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:100

For IF starting dilution is: 1:100
Background:
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.
Background References:
  • References for protein:
  • Torres,M.I., Pathol. Res. Pract. 203 (6), 485-487 (2007)
  • Olsen,L., Am. J. Physiol. Gastrointest. Liver Physiol. 289 (2), G220-G226(2005)
  • Alkhoury,F., Am. J. Physiol. Gastrointest. Liver Physiol. 289 (2), G285-G290(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Alkaline Phosphatase (ALPI) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-304 amino acids from the Central region of human Alkaline Phosphatase (ALPI).
NCBI Gene ID #:
248
NCBI Official Name:
Intestinal-type alkaline phosphatase
NCBI Official Symbol:
ALPI
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa
Protein Accession #:
P09923
Protein GI Number:
130744
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Cell Cycle,Immunology,Obesity,Signal Transduction
Swissprot #:
P09923
User NOte:
Optimal dilutions for each application to be determined by the researcher.