HNF4A Antibody

ProSci
Product Code: PSI-63-943
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-943-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western Blot at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.
2 / 3
Western Blot at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.

Further Information

Additional Names:
Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, TCF-14, Transcription factor HNF-4, HNF4A, HNF4, NR2A1, TCF14
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
HNF4A is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines.
Background References:
  • Koh, W., et al. BMC Genomics 11 SUPPL 1, S6 (2010)
  • Wang, F., et al. Chin. Med. J. 122(20):2477-2482(2009)
  • Taghavi, S.M., et al. Cardiovasc Diabetol 8, 63 (2009)
  • Guo, H., et al. Biochem. J. 394 (PT 2), 379-387 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Xenopus
Immunogen:
This HNF4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-139 amino acids from the N-terminal region of human HNF4A.
NCBI Gene ID #:
3172
NCBI Official Name:
Hepatocyte nuclear factor 4-alpha
NCBI Official Symbol:
HNF4A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
53 kDa
Protein Accession #:
P41235
Protein GI Number:
148886624
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P41235
User NOte:
Optimal dilutions for each application to be determined by the researcher.