HNF4G Antibody

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ProSci
Product Code: PSI-62-921
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-921-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in WiDr cell line lysates (35ug/lane).
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Formalin-fixed and paraffin-embedded human testis tissue reacted with HNF4G Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis in WiDr cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human testis tissue reacted with HNF4G Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Hepatocyte nuclear factor 4-gamma, HNF-4-gamma, Nuclear receptor subfamily 2 group A member 2, HNF4G, NR2A2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
HNF4G is a transcription factor. It has a lower transcription activation potential than HNF4-alpha.
Background References:
  • Plengvidhya N., Antonellis A.Diabetes 48:2099-2102(1999)
  • Drewes T., Senkel S.Mol. Cell. Biol. 16:925-931(1996)
  • Wisely G.B., Miller A.B.Structure 10:1225-1234(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HNF4G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-100 amino acids from the N-terminal region of human HNF4G.
NCBI Gene ID #:
3174
NCBI Official Name:
Hepatocyte nuclear factor 4-gamma
NCBI Official Symbol:
HNF4G
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46 kDa
Protein Accession #:
Q14541
Protein GI Number:
160110004
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Cell Cycle,Signal Transduction
Swissprot #:
Q14541
User NOte:
Optimal dilutions for each application to be determined by the researcher.