ALPP Antibody

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ProSci
Product Code: PSI-57-115
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-115-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Hela cell line lysates (35ug/lane).
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ALPP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in Hela cell line lysates (35ug/lane).
ALPP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Alkaline phosphatase, placental type, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1, ALPP, PLAP
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.
Background References:
  • Stec, B., et al. Acta Crystallogr. Sect.Struct. Biol. Cryst. Commun. 66 (PT 8), 866-870 (2010) :
  • Wang, F., et al. Am. J. Surg. Pathol. 33(10):1529-1539(2009)
  • Estrada, K., et al. Hum. Mol. Genet. 18(18):3516-3524(2009)
  • Zhu, J.F., et al. Zhonghua Wai Ke Za Zhi 47(5):381-384(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ALPP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 282-309 amino acids from the Central region of human ALPP.
NCBI Gene ID #:
250
NCBI Official Name:
Alkaline phosphatase, placental type
NCBI Official Symbol:
ALPP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
58 kDa
Protein Accession #:
P05187
Protein GI Number:
130737
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Immunology
Swissprot #:
P05187
User NOte:
Optimal dilutions for each application to be determined by the researcher.