SPB4 Antibody

ProSci
Product Code: PSI-55-921
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-921-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in K562 cell line lysates (35ug/lane).
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Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Serpin B4, Leupin, Peptidase inhibitor 11, PI-11, Squamous cell carcinoma antigen 2, SCCA-2, SERPINB4, PI11, SCCA2
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
SPB4 may act as a protease inhibitor to modulate the host immune response against tumor cells.
Background References:
  • Murakami, A., et al. Int. J. Oncol. 36(6):1395-1400(2010)
  • Higgins, W.J., et al. J. Biol. Chem. 285(6):3722-3729(2010)
  • Villano, G., et al. Int. J. Mol. Med. 25(1):137-143(2010)
  • Ahmed, S.T., et al. Biochem. Biophys. Res. Commun. 378(4):821-825(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SPB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 268-296 amino acids from the C-terminal region of human SPB4.
NCBI Gene ID #:
6318
NCBI Official Name:
Serpin B4
NCBI Official Symbol:
SERPINB4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
P48594
Protein GI Number:
1710877
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Immunology,
Swissprot #:
P48594
User NOte:
Optimal dilutions for each application to be determined by the researcher.