TPA Antibody
| Code | Size | Price |
|---|
| PSI-62-457-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Tissue-type plasminogen activator, t-PA, t-plasminogen activator, tPA, Alteplase, Reteplase, Tissue-type plasminogen activator chain A, Tissue-type plasminogen activator chain B, PLAT
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Background:
TPA is a tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism.
Background References:
- de Vos,A.M., et.al., Biochemistry 31 (1), 270-279 (1992)
- Bentov,Y., et.al., PLoS ONE(6), E5918 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 371-399 amino acids from the Central region of human TPA.
NCBI Gene ID #:
5327
NCBI Official Name:
Tissue-type plasminogen activator
NCBI Official Symbol:
PLAT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
63 kDa
Protein Accession #:
P00750
Protein GI Number:
137119
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Immunology,Obesity
Swissprot #:
P00750
User NOte:
Optimal dilutions for each application to be determined by the researcher.