PKC theta Antibody
| Code | Size | Price |
|---|
| PSI-62-601-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Protein kinase C theta type, nPKC-theta, PRKCQ, PRKCT
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IHC-P starting dilution is: 1:50~100
Background:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. PKC theta is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.
Background References:
- Carrasco, S., et al., Mol. Biol. Cell 15(6):2932-2942 (2004).
- Sun, L., et al., Blood 103(3):948-954 (2004).
- Burchfield, J.G., et al., J. Biol. Chem. 279(18):18623-18632 (2004).
- Ishaq, M., et al., J. Biol. Chem. 278(41):39296-39302 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PKC theta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-348 amino acids from the C-terminal region of human PKC theta.
NCBI Gene ID #:
5588
NCBI Official Name:
Protein kinase C theta type
NCBI Official Symbol:
PRKCQ
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
82 kDa
Protein Accession #:
Q04759
Protein GI Number:
20141582
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Immunology,Obesity,Signal Transduction
Swissprot #:
Q04759
User NOte:
Optimal dilutions for each application to be determined by the researcher.