PIK3CB Antibody

ProSci
Product Code: PSI-63-345
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-345-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PIK3CB antibody detected the PIK3CB protein (arrow).
2 / 3
Western blot analysis of PIK3CB using rabbit polyclonal P using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIK3CB gene.
3 / 3

Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PIK3CB antibody detected the PIK3CB protein (arrow).
Western blot analysis of PIK3CB using rabbit polyclonal P using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIK3CB gene.

Further Information

Additional Names:
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform, PI3-kinase subunit beta, PI3K-beta, PI3Kbeta, PtdIns-3-kinase subunit beta, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta, PtdIns-3-kinase subunit p110-beta, p110beta, PIK3CB, PIK3C1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PIK3CB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-154 amino acids from the N-terminal region of human PIK3CB.
NCBI Gene ID #:
5291
NCBI Official Name:
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
NCBI Official Symbol:
PIK3CB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
123 kDa
Protein Accession #:
P42338
Protein GI Number:
1171955
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Immunology,Signal Transduction
Swissprot #:
P42338
User NOte:
Optimal dilutions for each application to be determined by the researcher.