FUT6 Antibody

ProSci
Product Code: PSI-61-717
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-717-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 4
Western blot analysis of FUT6 Antibody in HL-60 cell line lysates (35ug/lane)
3 / 4
4 / 4
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of FUT6 Antibody in HL-60 cell line lysates (35ug/lane)
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Alpha-(1,3)-fucosyltransferase 6, Fucosyltransferase 6, Fucosyltransferase VI, Fuc-TVI, FucT-VI, Galactoside 3-L-fucosyltransferase, FUT6, FCT3A
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
FUT6 is a Golgi stack membrane protein that is involved in the creation of sialyl-Lewis X, an E-selectin ligand.
Background References:
  • Norden, R., et al. Glycobiology 19(7):776-788(2009)
  • Higai, K., et al. Glycoconj. J. 25(3):225-235(2008)
  • Martinez-Duncker, I., et al. Glycobiology 14(1):13-25(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This FUT6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-130 amino acids from the Central region of human FUT6.
NCBI Gene ID #:
2528
NCBI Official Name:
Alpha-(1,3)-fucosyltransferase 6
NCBI Official Symbol:
FUT6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
P51993
Protein GI Number:
1730136
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Immunology,Signal Transduction
Swissprot #:
P51993
User NOte:
Optimal dilutions for each application to be determined by the researcher.