RORA Antibody

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ProSci
Product Code: PSI-55-471
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-471-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-RORA Antibody (T216) in A2058 cell line lysates (35ug/lane)
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Fluorescent confocal image of Hela cell stained with RORA Antibody (T216).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RORA primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RORA immunoreactivity is localized to Nucleus significantly.
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Western blot analysis of anti-RORA Antibody (T216) in A2058 cell line lysates (35ug/lane)
Fluorescent confocal image of Hela cell stained with RORA Antibody (T216).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RORA primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RORA immunoreactivity is localized to Nucleus significantly.

Documents

Further Information

Additional Names:
Nuclear receptor ROR-alpha, Nuclear receptor RZR-alpha, Nuclear receptor subfamily 1 group F member 1, RAR-related orphan receptor A, Retinoid-related orphan receptor-alpha, RORA, NR1F1, RZRA
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
RORA is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The specific functions of this protein are not known, but it has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene.
Background References:
  • Lechtken,A., Biochem. Biophys. Res. Commun. 358 (3), 890-896 (2007)
  • Lanoix,D.,Hum. Reprod. 21 (8), 1981-1989 (2006)
  • Boukhtouche,F., J. Neurochem. 96 (6), 1778-1789 (2006)
  • Klar,J., Eur. J. Hum. Genet. 13 (8), 928-934 (2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This RORA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-222 amino acids from human RORA.
NCBI Gene ID #:
6095
NCBI Official Name:
Nuclear receptor ROR-alpha
NCBI Official Symbol:
RORA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
59 kDa
Protein Accession #:
P35398
Protein GI Number:
548814
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Neuroscience
Swissprot #:
P35398
User NOte:
Optimal dilutions for each application to be determined by the researcher.