IRX3 Antibody

ProSci
Product Code: PSI-57-809
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-809-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 2
IRX3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western Blot at 1:1000 dilution + SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
IRX3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Iroquois-class homeodomain protein IRX-3, Homeodomain protein IRXB1, Iroquois homeobox protein 3, IRX3, IRXB1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
IRX3 is a member of the Iroquois homeobox gene family (see IRX1; MIM 606197) and plays a role in an early step of neural development (Bellefroid et al., 1998 [PubMed 9427753]). Members of this family appear to play multiple roles during pattern formation of vertebrate embryos (Lewis et al., 1999 [PubMed 10370142]).
Background References:
  • Ragvin, A., et al. Proc. Natl. Acad. Sci. U.S.A. 107(2):775-780(2010)
  • Trynka, G., et al. Gut 58(8):1078-1083(2009)
  • Lewis, M.T., et al. Cell Tissue Res. 296(3):549-554(1999)
  • Bellefroid, E.J., et al. EMBO J. 17(1):191-203(1998)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Xenopus
Immunogen:
This IRX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the N-terminal region of human IRX3.
NCBI Gene ID #:
79191
NCBI Official Name:
Iroquois-class homeodomain protein IRX-3
NCBI Official Symbol:
IRX3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
52 kDa
Protein Accession #:
P78415
Protein GI Number:
47117874
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Neuroscience
Swissprot #:
P78415
User NOte:
Optimal dilutions for each application to be determined by the researcher.