EFNB2 Antibody

ProSci
Product Code: PSI-55-964
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-964-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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EFNB2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in Hela,293 cell line lysates (35ug/lane).
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EFNB2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Hela,293 cell line lysates (35ug/lane).

Further Information

Additional Names:
Ephrin-B2, EPH-related receptor tyrosine kinase ligand 5, LERK-5, HTK ligand, HTK-L, EFNB2, EPLG5, HTKL, LERK5
Application Note:
For IHC-P starting dilution is: 1:10~50

For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors.
Background References:
  • Zhang, R., et al. Psychiatry Res 180(1):5-9(2010)
  • Bochenek, M.L., et al. J. Cell. Sci. 123 (PT 8), 1235-1246 (2010) :
  • Nakada, M., et al. Int. J. Cancer 126(5):1155-1165(2010)
  • Qin, H., et al. J. Biol. Chem. 285(1):644-654(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This EFNB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 157-186 amino acids from the Central region of human EFNB2.
NCBI Gene ID #:
1948
NCBI Official Name:
Ephrin-B2
NCBI Official Symbol:
EFNB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
37 kDa
Protein Accession #:
P52799
Protein GI Number:
1706673
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Neuroscience
Swissprot #:
P52799
User NOte:
Optimal dilutions for each application to be determined by the researcher.