KLF5 Antibody

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ProSci
Product Code: PSI-62-839
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-839-400ul400ul£662.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Western blot analysis in CEM cell line lysates (35ug/lane).
2 / 4
Fluorescent confocal image of U251 cell stained with KLF5 Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF5 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). KLF5 immunoreactivity is localized to vesicles and Nucleus significantly.
3 / 4
4 / 4
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in CEM cell line lysates (35ug/lane).
Fluorescent confocal image of U251 cell stained with KLF5 Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF5 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). KLF5 immunoreactivity is localized to vesicles and Nucleus significantly.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Krueppel-like factor 5, Basic transcription element-binding protein 2, BTE-binding protein 2, Colon krueppel-like factor, GC-box-binding protein 2, Intestinal-enriched krueppel-like factor, Transcription factor BTEB2, KLF5, BTEB2, CKLF, IKLF
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
KLF5 is a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, the protein is thought to be a transcription factor.
Background References:
  • Guo,P., Dong,X.Y. J. Biol. Chem. 284 (10), 6071-6078 (2009)
  • Lee,M.Y., Moon,J.S. Biochem. J. 417 (1), 313-322 (2009)
  • Du,J.X., Bialkowska,A.B. J. Biol. Chem. 283 (46), 31991-32002 (2008)
  • Miyamoto,S., Suzuki,T. Mol. Cell. Biol. 23 (23), 8528-8541 (2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This KLF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-370 amino acids from the C-terminal region of human KLF5.
NCBI Gene ID #:
688
NCBI Official Name:
Krueppel-like factor 5
NCBI Official Symbol:
KLF5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
51 kDa
Protein Accession #:
Q13887
Protein GI Number:
12644412
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Neuroscience,Signal Transduction
Swissprot #:
Q13887
User NOte:
Optimal dilutions for each application to be determined by the researcher.