IGF1R Antibody

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ProSci
Product Code: PSI-63-098
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-098-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of IGF1R Antibody (N-term K66) in WiDr cell line lysates (35ug/lane)
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IGF1R Antibody (N-term K66) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IGF1R Antibody (N-term K66) for immunohistochemistry.
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Confocal immunofluorescent analysis of IGF1R Antibody (N-term K66) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of IGF1R Antibody (N-term K66) in WiDr cell line lysates (35ug/lane)
IGF1R Antibody (N-term K66) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IGF1R Antibody (N-term K66) for immunohistochemistry.
Confocal immunofluorescent analysis of IGF1R Antibody (N-term K66) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, IGF-I receptor, CD221, Insulin-like growth factor 1 receptor alpha chain, Insulin-like growth factor 1 receptor beta chain, IGF1R
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
The IGF1R receptor binds insulin-like growth factor with a high affinity and plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. The protein possess tyrosine kinase activity.
Background References:
  • Song, R.X., et al., Proc. Natl. Acad. Sci. U.S.A. 101(7):2076-2081 (2004).
  • Zhao, H., et al., Oncogene 23(3):786-794 (2004).
  • Lu, Y., et al., Biochem. Biophys. Res. Commun. 313(3):709-715 (2004).
  • Hakam, A., et al., Dig. Dis. Sci. 48(10):1972-1978 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This IGF1R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-77 amino acids from the N-terminal region of human IGF1R.
NCBI Gene ID #:
3480
NCBI Official Name:
Insulin-like growth factor 1 receptor
NCBI Official Symbol:
IGF1R
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
155 kDa
Protein Accession #:
P08069
Protein GI Number:
124240
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Neuroscience,Signal Transduction
Swissprot #:
P08069
User NOte:
Optimal dilutions for each application to be determined by the researcher.