Sestrin-3 Antibody

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ProSci
Product Code: PSI-56-448
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-448-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Sestrin-3 antibody detected the Sestrin-3 protein (arrow).
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Sest immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Sest for immunohistochemistry.
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Western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Sestrin-3 antibody detected the Sestrin-3 protein (arrow).
Sest immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Sest for immunohistochemistry.

Documents

Further Information

Additional Names:
Sestrin-3, SESN3, SEST3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Sestrins form a small family of redox enzymes which regulate accumulation of Reactive Oxygen Species. They protect cells and their DNA against oxidative damage and regulate cell growth and viability. These genes are often deregulated in cancers and their inactivation accelerates the growth of model tumors in mice.
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Chen, C.C., et al. Dev. Cell 18(4):592-604(2010)
  • Peeters, H., et al. Hum. Genet. 112 (5-6), 573-580 (2003) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This Sestrin-3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 320-347 amino acids from the Central region of human Sestrin-3.
NCBI Gene ID #:
143686
NCBI Official Name:
Sestrin-3
NCBI Official Symbol:
SESN3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa
Protein Accession #:
P58005
Protein GI Number:
20141774
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Obesity
Swissprot #:
P58005
User NOte:
Optimal dilutions for each application to be determined by the researcher.