eNos Antibody

ProSci
Product Code: PSI-56-064
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-064-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in K562 cell line lysates (35ug/lane).
2 / 3
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
3 / 3

Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Nitric oxide synthase, endothelial, Constitutive NOS, cNOS, EC-NOS, Endothelial NOS, eNOS, NOS type III, NOSIII, NOS3
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene.
Background References:
  • Yanamandra, K., et al. Ophthalmic Genet. 31(4):173-177(2010)
  • Bambha, K., et al. Mayo Clin. Proc. 85(9):814-820(2010)
  • Shin, S.J., et al. Eur. J. Obstet. Gynecol. Reprod. Biol. 152(1):64-67(2010)
  • Kim, S.M., et al. Kardiol Pol 68(8):920-926(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat
Immunogen:
This eNos antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1156-1183 amino acids from human eNos.
NCBI Gene ID #:
4846
NCBI Official Name:
Nitric oxide synthase, endothelial
NCBI Official Symbol:
NOS3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
133 kDa
Protein Accession #:
P29474
Protein GI Number:
266648
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Obesity,Neuroscience
Swissprot #:
P29474
User NOte:
Optimal dilutions for each application to be determined by the researcher.