CASP12 Antibody
| Code | Size | Price |
|---|
| PSI-56-185-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Inactive caspase-12, CASP-12, CASP12
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Background:
Caspases are cysteine proteases that cleave C-terminal
aspartic acid residues on their substrate molecules. This gene is
most highly related to members of the ICE subfamily of caspases
that process inflammatory cytokines. In rodents, the homolog of
this gene mediates apoptosis in response to endoplasmic reticulum
stress. However, in humans this gene contains a polymorphism for
the presence or absence of a premature stop codon. The majority of
human individuals have the premature stop codon and produce a
truncated non-functional protein. The read-through codon occurs
primarily in individuals of African descent and carriers have
endotoxin hypo-responsiveness and an increased susceptibility to
severe sepsis. Several alternatively spliced transcript variants
have been noted for this gene.
Background References:
- Lee, H.J., et al. Arch. Biochem. Biophys. 502(1):68-73(2010)
- Plantinga, T.S., et al. J. Acquir. Immune Defic. Syndr. 55(1):87-94(2010)
- McCall, M.B., et al. Eur. Cytokine Netw. 21(2):77-83(2010)
- Kachapati, K., et al. Hum. Mutat. 27 (9), 975 (2006) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CASP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-193 amino acids from the Central region of human CASP12.
NCBI Gene ID #:
100506742
NCBI Official Name:
Inactive caspase-12
NCBI Official Symbol:
CASP12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
Q6UXS9
Protein GI Number:
395398427
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Obesity,Neuroscience,
Swissprot #:
Q6UXS9
User NOte:
Optimal dilutions for each application to be determined by the researcher.