SDHA Antibody

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ProSci
Product Code: PSI-60-990
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-60-990-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using SDHA Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Western blot analysis in Hela and mouse C2C12 cell line lysates (35ug/lane).
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Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using SDHA Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis in Hela and mouse C2C12 cell line lysates (35ug/lane).

Documents

Further Information

Additional Names:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial, Flavoprotein subunit of complex II, Fp, SDHA, SDH2, SDHF
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.
Background References:
  • Hirawake H., et al. J. Biochem. 116:221-227(1994).
  • Morris A.A.M., et al. Biochim. Biophys. Acta 1185:125-128(1994).
  • Parfait B., et al. Hum. Genet. 106:236-243(2000).
  • Ota T., et al. Nat. Genet. 36:40-45(2004).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Rat
Immunogen:
This SDHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 577-605 amino acids from the C-terminal region of human SDHA.
NCBI Gene ID #:
6389
NCBI Official Name:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
NCBI Official Symbol:
SDHA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
73 kDa
Protein Accession #:
P31040
Protein GI Number:
1169337
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P31040
User NOte:
Optimal dilutions for each application to be determined by the researcher.