ProSci

HSPA6 Antibody

Product Code:
 
PSI-63-604
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of lysate from Jurkat cell line,using HSPA6 Antibody .AP8708b was diluted at 1:1000 at each lane
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Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HSPA6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of lysate from Jurkat cell line,using HSPA6 Antibody .AP8708b was diluted at 1:1000 at each lane
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HSPA6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-604-400ul400ul£626.00
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Further Information

Additional Names:
Heat shock 70 kDa protein 6, Heat shock 70 kDa protein B', HSPA6, HSP70B'
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
Background References:
  • Leung,T.K., et.al.,Genomics 12 (1), 74-79 (1992)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HSPA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 544-571 amino acids from the C-terminal region of human HSPA6.
NCBI Gene ID #:
3310
NCBI Official Name:
Heat shock 70 kDa protein 6
NCBI Official Symbol:
HSPA6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
71 kDa
Protein Accession #:
P17066
Protein GI Number:
34978357
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P17066
User NOte:
Optimal dilutions for each application to be determined by the researcher.