AMY1A Antibody

ProSci
Product Code: PSI-63-984
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-984-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of AMY1A Antibody in HL-60 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with AMY1A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of AMY1A Antibody in HL-60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with AMY1A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Alpha-amylase 1,4-alpha-D-glucan glucanohydrolase 1, Salivary alpha-amylase, AMY1A, AMY1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. It is an amylase isoenzyme produced by the salivary gland.
Background References:
  • Ragunath, C., et al. J. Mol. Biol. 384(5):1232-1248(2008)
  • Takahashi, T., et al. Neuro Endocrinol. Lett. 28(5):662-665(2007)
  • Perry, G.H., et al. Nat. Genet. 39(10):1256-1260(2007)
  • Chaudhuri, B., et al. BMC Microbiol. 7, 60 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig
Immunogen:
This AMY1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-223 amino acids from the Central region of human AMY1A.
NCBI Gene ID #:
276, 277, 278
NCBI Official Name:
Alpha-amylase 1
NCBI Official Symbol:
AMY1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
58 kDa
Protein Accession #:
P04745
Protein GI Number:
1351933
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P04745
User NOte:
Optimal dilutions for each application to be determined by the researcher.