PRDX6 Antibody

ProSci
Product Code: PSI-61-605
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-605-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of PRDX6 Antibody in CEM cell line lysates (35ug/lane)
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Western blot analysis of PRDX6 using rabbit polyclonal PRDX6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRDX6 gene (Lane 2).
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Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of PRDX6 Antibody in CEM cell line lysates (35ug/lane)
Western blot analysis of PRDX6 using rabbit polyclonal PRDX6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRDX6 gene (Lane 2).
Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Peroxiredoxin-6, 1-Cys peroxiredoxin, 1-Cys PRX, 24 kDa protein, Acidic calcium-independent phospholipase A2, aiPLA2, 311-, Antioxidant protein 2, Liver 2D page spot 40, Non-selenium glutathione peroxidase, NSGPx, Red blood cells page spot 12, PRDX6, AOP2, KIAA0106
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
PRDX6is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
Background References:
  • Sorokina,E.M., et.al., Am. J. Physiol. Lung Cell Mol. Physiol. 297 (5), L871-L880 (2009)
  • Manevich,Y., et.al., Arch. Biochem. Biophys. 485 (2), 139-149 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Monkey, Mouse, Pig, Rat
Immunogen:
This PRDX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 197-224 amino acids from the C-terminal region of human PRDX6.
NCBI Gene ID #:
9588
NCBI Official Name:
Peroxiredoxin-6
NCBI Official Symbol:
PRDX6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
25 kDa
Protein Accession #:
P30041
Protein GI Number:
1718024
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P30041
User NOte:
Optimal dilutions for each application to be determined by the researcher.