CALM1 Antibody

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ProSci
Product Code: PSI-63-511
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-511-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of CALM1 Antibody in Hela cell line lysates (35ug/lane)(2ug/ml)
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Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Confocal immunofluorescent analysis of CALM1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of CALM1 Antibody in Hela cell line lysates (35ug/lane)(2ug/ml)
Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of CALM1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Calmodulin, CaM, CALM1, CALM, CAM, CAM1
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50
Background:
CALM1 is a member of the EF-hand calcium-binding protein family.Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
Background References:
  • Zhao,D., et.al., Zhonghua Yi Xue Za Zhi 88 (35), 2452-2456 (2008)
  • Martins-de-Souza,D., et.al., J. Neural Transm. 116 (3), 275-289 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, C. Elegans, Chicken, Zebrafish, Drosophila, Mouse, Rabbit, Rat, Xenopus
Immunogen:
This CALM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-132 amino acids from the C-terminal region of human CALM1.
NCBI Gene ID #:
801, 805, 808
NCBI Official Name:
Calmodulin
NCBI Official Symbol:
CALM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
17 kDa
Protein Accession #:
P62158
Protein GI Number:
49037474
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P62158
User NOte:
Optimal dilutions for each application to be determined by the researcher.