NEK2 Antibody
| Code | Size | Price |
|---|
| PSI-63-379-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Serine/threonine-protein kinase Nek2, HSPK 21, Never in mitosis A-related kinase 2, NimA-related protein kinase 2, NimA-like protein kinase 1, NEK2, NEK2A, NLK1
Application Note:
For WB starting dilution is: 1:1000
Background:
NEK2 is closely related in its catalytic domain to the serine/threonine
protein kinase NIMA of Aspergillus nidulans which is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulates progressively throughout S, reaching maximal levels in late G2. These observations demonstrate that NEK2 resembles Aspergillus NIMA, both in its catalytic domain, and cell cycle-dependent expression. Recombinant NEK2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation. Both human NEK2 and fungal NIMA phosphorylate a similar, but not identical, set of proteins and synthetic peptides. NEK2 is shown to be expressed most abundantly in adult testis and there is increasing evidence that NEK2 is abnormally expressed in a wide variety of human cancers. This antibody is specific to isoform 2 of NEK2.
Background References:
- Blume-Jensen P, et al. Nature 2001. 411: 355.
- Cantrell D, J. Cell Sci. 2001. 114: 1439.
- JhiangOncogene 2000. 19: 5590.
- Manning G, et al. Science 2002. 298: 1912.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 350-382 amino acids from the Central region of human NEK2.
NCBI Gene ID #:
4751
NCBI Official Name:
Serine/threonine-protein kinase Nek2
NCBI Official Symbol:
NEK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
52 kDa
Protein Accession #:
P51955
Protein GI Number:
1709252
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
P51955
User NOte:
Optimal dilutions for each application to be determined by the researcher.