Neutrophil elastase Antibody
| Code | Size | Price |
|---|
| PSI-56-220-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Neutrophil elastase, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, HLE, Medullasin, PMN elastase, ELANE, ELA2
Application Note:
For WB starting dilution is: 1:1000
Background:
Elastases form a subfamily of serine proteases that
hydrolyze many proteins in addition to elastin. Humans have six
elastase genes which encode the structurally similar proteins. The
product of this gene hydrolyzes proteins within specialized
neutrophil lysosomes, called azurophil granules, as well as
proteins of the extracellular matrix following the protein's
release from activated neutrophils. The enzyme may play a role in
degenerative and inflammatory diseases by its proteolysis of
collagen-IV and elastin of the extracellular matrix. This protein
degrades the outer membrane protein A (OmpA) of E. coli as well as
the virulence factors of such bacteria as Shigella, Salmonella and
Yersinia. Mutations in this gene are associated with cyclic
neutropenia and severe congenital neutropenia (SCN). This gene is
clustered with other serine protease gene family members,
azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3
genes are expressed coordinately and their protein products are
packaged together into azurophil granules during neutrophil
differentiation.
Background References:
- Kallquist, L., et al. Exp. Cell Res. 316(19):3182-3196(2010)
- Rabai, G., et al. Thromb. Res. 126 (2), E94-E101 (2010) :
- Newburger, P.E., et al. Pediatr Blood Cancer 55(2):314-317(2010)
- Hayashi, M., et al.Nippon Med Sch 77(2):80-85(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Neutrophil elastase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-39 amino acids from the N-terminal region of human Neutrophil elastase.
NCBI Gene ID #:
1991
NCBI Official Name:
Neutrophil elastase
NCBI Official Symbol:
ELANE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
29 kDa
Protein Accession #:
P08246
Protein GI Number:
119292
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
P08246
User NOte:
Optimal dilutions for each application to be determined by the researcher.