SMAD5 Antibody

ProSci
Product Code: PSI-60-724
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-60-724-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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SMAD5 Antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide in Hela cell line lysate. SMAD5 Antibody (arrow) was detected using the purified Pab.

SMAD5 Antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide in Hela cell line lysate. SMAD5 Antibody (arrow) was detected using the purified Pab.

Further Information

Additional Names:
Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5, SMAD 5, Smad5, hSmad5, SMAD5, MADH5
Application Note:
For WB starting dilution is: 1:1000
Background:
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Background References:
  • Yang, J., et al. Circ. Res. 107(2):252-262(2010)
  • Tao, S., et al. Dev. Growth Differ. 52(4):335-342(2010)
  • Middlebrook, B.S., et al. Endocrinology 150(12):5208-5217(2009)
  • Su, D., et al. J. Biol. Chem. 284(18):12153-12164(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This SMAD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-269 amino acids from the Central region of human SMAD5.
NCBI Gene ID #:
4090
NCBI Official Name:
Mothers against decapentaplegic homolog 5
NCBI Official Symbol:
SMAD5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
52 kDa
Protein Accession #:
Q99717
Protein GI Number:
13959566
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
Q99717
User NOte:
Optimal dilutions for each application to be determined by the researcher.