MMP15 Antibody

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ProSci
Product Code: PSI-57-244
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-244-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in HL-60 cell line lysates (35ug/lane).
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MMP15 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in HL-60 cell line lysates (35ug/lane).
MMP15 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Matrix metalloproteinase-15, MMP-15, 3424-, Membrane-type matrix metalloproteinase 2, MT-MMP 2, MTMMP2, Membrane-type-2 matrix metalloproteinase, MT2-MMP, MT2MMP, SMCP-2, MMP15
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted.
Background References:
  • Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Nalpas, B., et al. Gut 59(8):1120-1126(2010)
  • Romero, R., et al. Am. J. Obstet. Gynecol. 202 (5), 431 (2010) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MMP15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-196 amino acids from the N-terminal region of human MMP15.
NCBI Gene ID #:
4324
NCBI Official Name:
Matrix metalloproteinase-15
NCBI Official Symbol:
MMP15
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
76 kDa
Protein Accession #:
P51511
Protein GI Number:
1705988
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
P51511
User NOte:
Optimal dilutions for each application to be determined by the researcher.