CAMK1D Antibody

ProSci
Product Code: PSI-62-729
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-729-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Western blot analysis of anti-CAMK1-like C-term Pab in NCIH460 cell lysate. CAMK1-like was detected using purified Pab.
2 / 4
Western blot analysis of CAMK1D using CAMK1D (CAMK1 delta)Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK1D gene (Lane 2).
3 / 4
4 / 4
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CAMK1-like Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot analysis of anti-CAMK1-like C-term Pab in NCIH460 cell lysate. CAMK1-like was detected using purified Pab.
Western blot analysis of CAMK1D using CAMK1D (CAMK1 delta)Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK1D gene (Lane 2).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CAMK1-like Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Calcium/calmodulin-dependent protein kinase type 1D, CaM kinase I delta, CaM kinase ID, CaM-KI delta, CaMKI delta, CaMKID, CaMKI-like protein kinase, CKLiK, CAMK1D, CAMKID
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
CAMK1-like protein kinase belongs to a proposed calcium-triggered signaling cascade. This protein is expressed in polymorphonuclear leukocytes (PMNs) and may be part of the chemokine signal transduction pathway that regulates granulocyte function. CAMK1-like protein kinase may play a role in apoptosis of erythroleukemia cells. It activates MAP kinase MAPK3, and in vitro, phosphorylates transcription factor CREM isoform Beta and probably CREB1.
Background References:
  • Verploegen, S., et al., Blood 96(9):3215-3223 (2000).
  • Ishikawa, Y., et al., FEBS Lett. 550 (1-3), 57-63 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CAMK1D (CAMK1 delta) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-333 amino acids from the C-terminal region of human CAMK1D (CAMK1 delta).
NCBI Gene ID #:
57118
NCBI Official Name:
Calcium/calmodulin-dependent protein kinase type 1D
NCBI Official Symbol:
CAMK1D
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
43 kDa
Protein Accession #:
Q8IU85
Protein GI Number:
56404610
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
Q8IU85
User NOte:
Optimal dilutions for each application to be determined by the researcher.