MMP3 Antibody

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ProSci
Product Code: PSI-62-179
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-179-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: U118MG whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MMP3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western Blot at 1:1000 dilution Lane 1: U118MG whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MMP3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Stromelysin-1, SL-1, Matrix metalloproteinase-3, MMP-3, Transin-1, MMP3, STMY1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP3 is an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation.
Background References:
  • Sage, E.H., et al., J. Biol. Chem. 278(39):37849-37857 (2003).
  • Matsuyama, A., et al., Circulation 108(12):1469-1473 (2003).
  • Mercapide, J., et al., Int. J. Cancer 106(5):676-682 (2003).
  • Bodemer, C., et al., J. Invest. Dermatol. 121(2):273-279 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-327 amino acids from the Central region of human MMP3.
NCBI Gene ID #:
4314
NCBI Official Name:
Stromelysin-1
NCBI Official Symbol:
MMP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
P08254
Protein GI Number:
116857
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
P08254
User NOte:
Optimal dilutions for each application to be determined by the researcher.