KRT13 Antibody

ProSci
Product Code: PSI-62-415
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-415-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of KRT13 antibody in 293 cell line lysates (35ug/lane)
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KRT13 Antibody (RB18903) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of KRT13 antibody in 293 cell line lysates (35ug/lane)
KRT13 Antibody (RB18903) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Keratin, type I cytoskeletal 13, Cytokeratin-13, CK-13, Keratin-13, K13, KRT13
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
KRT13 is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in its gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus.
Background References:
  • Sheng,S., Mol. Cell. Endocrinol. 296 (1-2), 1-9 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This KRT13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-138 amino acids from the N-terminal region of human KRT13.
NCBI Gene ID #:
3860
NCBI Official Name:
Keratin, type I cytoskeletal 13
NCBI Official Symbol:
KRT13
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
P13646
Protein GI Number:
269849755
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
P13646
User NOte:
Optimal dilutions for each application to be determined by the researcher.