HRAS Antibody

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ProSci
Product Code: PSI-63-189
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-189-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysate from 293T cell line, using HRAS Antibody at 1:1000.
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Western blot analysis of anti-HRAS Antibody in Jurkat cell line lysates (35ug/lane)
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Confocal immunofluorescent analysis of HRAS Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of lysate from 293T cell line, using HRAS Antibody at 1:1000.
Western blot analysis of anti-HRAS Antibody in Jurkat cell line lysates (35ug/lane)
Confocal immunofluorescent analysis of HRAS Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
GTPase HRas, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras, GTPase HRas, N-terminally processed, HRAS, HRAS1
Application Note:
For WB starting dilution is: 1:500

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
HRAS belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. These proteins function in signal transduction pathways. They can bind GTP and GDP, and they have intrinsic GTPase activity. HRAS undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in the HRAS gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma.
Background References:
  • Winter-Vann,A.M., Proc. Natl. Acad. Sci. U.S.A. 100 (11), 6529-6534 (2003)
  • Coats,S.G., Biochemistry 38 (39), 12926-12934 (1999)
  • Sakai,E., Int. J. Cancer 52 (6), 867-872 (1992)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This HRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-128 amino acids from the Central region of human HRAS.
NCBI Gene ID #:
3265
NCBI Official Name:
GTPase HRas
NCBI Official Symbol:
HRAS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
21 kDa
Protein Accession #:
P01112
Protein GI Number:
131869
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
P01112
User NOte:
Optimal dilutions for each application to be determined by the researcher.