p53 Antibody
| Code | Size | Price |
|---|
| PSI-62-206-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53
Application Note:
For WB starting dilution is: 1:1000-1:2000
Background:
Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.
Background References:
- Blanchette, P., et al., Mol. Cell. Biol. 24(21):9619-9629 (2004).
- Adachi, K., et al., Oncogene 23(47):7791-7798 (2004).
- Zhang, Y., et al., J. Biol. Chem. 279(41):42545-42551 (2004).
- Anazawa, Y., et al., Oncogene 23(46):7621-7627 (2004).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This p53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 354-385 amino acids from the C-terminal region of human p53.
NCBI Gene ID #:
7157
NCBI Official Name:
Cellular tumor antigen p53
NCBI Official Symbol:
TP53
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
44 kDa
Protein Accession #:
P04637
Protein GI Number:
269849759
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Signal Transduction,
Swissprot #:
P04637
User NOte:
Optimal dilutions for each application to be determined by the researcher.