IL4R Antibody

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ProSci
Product Code: PSI-61-028
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-028-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Flow cytometric analysis of Ramos cells using IL4R Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
2 / 2
Western blot analysis of lysates from 293T, A431 cell line (from left to right), using IL4R Antibody at 1:1000 at each lane.
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Flow cytometric analysis of Ramos cells using IL4R Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysates from 293T, A431 cell line (from left to right), using IL4R Antibody at 1:1000 at each lane.

Documents

Further Information

Additional Names:
Interleukin-4 receptor subunit alpha, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4RA, CD124, Soluble interleukin-4 receptor subunit alpha, Soluble IL-4 receptor subunit alpha, Soluble IL-4R-alpha, sIL4Ralpha/prot, IL-4-binding protein, IL4-BP, IL4R, IL4RA
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.
Background References:
  • Idzerda R.L.,et al.J. Exp. Med. 171:861-873(1990).
  • Galizzi J.-P.,et al.Int. Immunol. 2:669-675(1990).
  • Kruse S.,et al.Int. Immunol. 11:1965-1970(1999).
  • Loftus B.J.,et al.Genomics 60:295-308(1999).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This IL4R antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 677-691 amino acids from the C-terminal region of human IL4R.
NCBI Gene ID #:
3566
NCBI Official Name:
Interleukin-4 receptor subunit alpha
NCBI Official Symbol:
IL4R
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
90 kDa
Protein Accession #:
P24394
Protein GI Number:
124335
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Immunology
Swissprot #:
P24394
User NOte:
Optimal dilutions for each application to be determined by the researcher.