REG3A Antibody

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ProSci
Product Code: PSI-61-054
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-054-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Flow cytometric analysis of HepG2 cells using REG3A Antibody (green) compared to an isotype control of rabbit IgG(blue). AP20597c was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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Western blot analysis of lysate from human pancreas tissue lysate, using REG3A Antibody at 1:1000.
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Flow cytometric analysis of HepG2 cells using REG3A Antibody (green) compared to an isotype control of rabbit IgG(blue). AP20597c was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysate from human pancreas tissue lysate, using REG3A Antibody at 1:1000.

Documents

Further Information

Additional Names:
Regenerating islet-derived protein 3-alpha, REG-3-alpha, Hepatointestinal pancreatic protein, HIP/PAP, Human proislet peptide, Pancreatitis-associated protein 1, Regenerating islet-derived protein III-alpha, Reg III-alpha, Regenerating islet-derived protein 3-alpha 165 kDa form, Regenerating islet-derived protein 3-alpha 15 kDa form, REG3A, HIP, PAP, PAP1
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.
Background References:
  • Itoh T.,et al.Biochim. Biophys. Acta 1172:184-186(1993).
  • Orelle B.,et al.J. Clin. Invest. 90:2284-2291(1992).
  • Lasserre C.,et al.Cancer Res. 52:5089-5095(1992).
  • Dusetti N.J.,et al.Genomics 19:108-114(1994).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This REG3A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 129-163 amino acids from the C-terminal region of human REG3A.
NCBI Gene ID #:
5068
NCBI Official Name:
Regenerating islet-derived protein 3-alpha
NCBI Official Symbol:
REG3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
19 kDa
Protein Accession #:
Q06141
Protein GI Number:
464341
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Immunology
Swissprot #:
Q06141
User NOte:
Optimal dilutions for each application to be determined by the researcher.