IL12_2 Antibody

Contact us
ProSci
Product Code: PSI-55-341
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-341-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Overlay histogram showing A431 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
2 / 4
Western Blot at 1:2000 dilution Lane 1: THP-1 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
3 / 4
4 / 4
IL12RB2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsils tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
❮❯

Overlay histogram showing A431 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution Lane 1: THP-1 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
IL12RB2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsils tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Interleukin-12 receptor subunit beta-2, IL-12 receptor subunit beta-2, IL-12R subunit beta-2, IL-12R-beta-2, IL-12RB2, IL12RB2
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense.
Background References:
  • Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Liu, X., et al. Nat. Genet. 42(8):658-660(2010)
  • Mizuki, N., et al. Nat. Genet. 42(8):703-706(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This IL12_2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 756-783 amino acids from the C-terminal region of human IL12_2.
NCBI Gene ID #:
3595
NCBI Official Name:
Interleukin-12 receptor subunit beta-2
NCBI Official Symbol:
IL12RB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
97 kDa
Protein Accession #:
Q99665
Protein GI Number:
12229836
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Immunology
Swissprot #:
Q99665
User NOte:
Optimal dilutions for each application to be determined by the researcher.