CD63 Antibody

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ProSci
Product Code: PSI-61-885
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-885-200ul200ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody staining CD63 in human melanoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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Overlay histogram showing HL-60 cells stained with Antibody (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Western Blot at 1:2000 dilution Lane 1: A2058 whole cell lysates Lane 2: A375 whole cell lysates Lane 3: HL-60 whole cell lysates Lane 4: THP-1 whole cell lysates Lane 5: U-87 MG whole cell lysates Lysates/proteins at 20 ug per lane.
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Western Blot at 1:1000 dilution Lane 1: A2058 whole cell lysates Lane 2: MDA-MB-453 whole cell lysates Lysates/proteins at 20 ug per lane.
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CD63 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Antibody staining CD63 in human melanoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Overlay histogram showing HL-60 cells stained with Antibody (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution Lane 1: A2058 whole cell lysates Lane 2: A375 whole cell lysates Lane 3: HL-60 whole cell lysates Lane 4: THP-1 whole cell lysates Lane 5: U-87 MG whole cell lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution Lane 1: A2058 whole cell lysates Lane 2: MDA-MB-453 whole cell lysates Lysates/proteins at 20 ug per lane.
CD63 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, CD63, CD63, MLA1, TSPAN30
Application Note:
For IHC-P starting dilution is: 1:25

For FACS starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
CD63 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. The use of alternate polyadenylation sites has been found for this gene.
Background References:
  • Weng, J., et al. J. Virol. 83(15):7467-7474(2009)
  • Kassahn, D., et al. Cell Death Differ. 16(1):115-124(2009)
  • Logozzi, M., et al. PLoS ONE(4), E5219 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CD63 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-190 amino acids from the C-terminal region of human CD63.
NCBI Gene ID #:
967
NCBI Official Name:
CD63 antigen
NCBI Official Symbol:
CD63
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
26 kDa
Protein Accession #:
P08962
Protein GI Number:
116026
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Immunology
Swissprot #:
P08962
User NOte:
Optimal dilutions for each application to be determined by the researcher.