MAPK8 Antibody

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ProSci
Product Code: PSI-55-516
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-516-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysate from Hela cell line, using JNK1 antibody at 1:1000 at each lane.
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JNK1-T183/Y185 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LJNK1-T183/Y185 antibody for immunohistochemistry.
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Western blot analysis of lysate from Hela cell line, using JNK1 antibody at 1:1000 at each lane.
JNK1-T183/Y185 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LJNK1-T183/Y185 antibody for immunohistochemistry.

Documents

Further Information

Additional Names:
Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, SAPK1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, MAPK8, JNK1, PRKM8, SAPK1, SAPK1C
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
Background References:
  • Dasgupta, J., et al. J. Cell. Physiol. 225(1):52-62(2010)
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Gao, D., et al. J. Biol. Chem. 285(39):29965-29973(2010)
  • Han, J.S., et al. Anticancer Res. 30(9):3407-3412(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Xenopus
Immunogen:
This MAPK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-195 amino acids from human MAPK8.
NCBI Gene ID #:
5599
NCBI Official Name:
Mitogen-activated protein kinase 8
NCBI Official Symbol:
MAPK8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
P45983
Protein GI Number:
2507195
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Immunology,Neuroscience,Signal Transduction
Swissprot #:
P45983
User NOte:
Optimal dilutions for each application to be determined by the researcher.