VEGFR2 Antibody

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ProSci
Product Code: PSI-63-092
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-092-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-KDR/FLK1 Pab in HeLa cell lysate. KDR (VEGFR2) was detected using purified Pab.
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Confocal immunofluorescent analysis of VEGFR2 (FLK1/KDR) Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Western blot analysis of anti-KDR/FLK1 Pab in HeLa cell lysate. KDR (VEGFR2) was detected using purified Pab.
Confocal immunofluorescent analysis of VEGFR2 (FLK1/KDR) Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Documents

Further Information

Additional Names:
Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, FLK-1, Kinase insert domain receptor, KDR, Protein-tyrosine kinase receptor flk-1, CD309, KDR, FLK1, VEGFR2
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:50~100
Background:
KDR (FLK1) is a receptor for VEGF or VEGFC. This protein has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.
Background References:
  • Yilmaz, A., et al., Biochem. Biophys. Res. Commun. 306(3):730-736 (2003).
  • Zeng, H., et al., J. Biol. Chem. 278(23):20738-20745 (2003).
  • Kiba, A., et al., J. Biol. Chem. 278(15):13453-13461 (2003).
  • Elvert, G., et al., J. Biol. Chem. 278(9):7520-7530 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This VEGFR2 (FLK1/KDR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1326-1356 amino acids from the C-terminal region of human VEGFR2 (FLK1/KDR).
NCBI Gene ID #:
3791
NCBI Official Name:
Vascular endothelial growth factor receptor 2
NCBI Official Symbol:
KDR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
152 kDa
Protein Accession #:
P35968
Protein GI Number:
9087218
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Immunology,Obesity,Signal Transduction
Swissprot #:
P35968
User NOte:
Optimal dilutions for each application to be determined by the researcher.