PI3KCG Antibody

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ProSci
Product Code: PSI-63-450
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-450-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of PI3KCG Antibody Pab using purified Pab using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PI3KCG gene (Lane 2).
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Formalin-fixed and paraffin-embedded skeletal muscle tissue reacted with PI3KCG antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of PI3KCG Antibody Pab using purified Pab using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PI3KCG gene (Lane 2).
Formalin-fixed and paraffin-embedded skeletal muscle tissue reacted with PI3KCG antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform, PI3-kinase subunit gamma, PI3K-gamma, PI3Kgamma, PtdIns-3-kinase subunit gamma, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma, PtdIns-3-kinase subunit p110-gamma, p110gamma, Phosphoinositide-3-kinase catalytic gamma polypeptide, Serine/threonine protein kinase PIK3CG, p120-PI3K, PIK3CG
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
PIK3CG belongs to the pi3/pi4-kinase family of proteins. It is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene for this protein is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias.
Background References:
  • Andreozzi, F., et al., Endocrinology 145(6):2845-2857 (2004).
  • Reddy, S.A., et al., Biochem. Biophys. Res. Commun. 316(4):1022-1028 (2004).
  • Osaki, M., et al., J. Cancer Res. Clin. Oncol. 130(1):8-14 (2004).
  • Khan, N.A., et al., J. Neurovirol. 9(6):584-593 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PI3KCG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 518-547 amino acids from the Central region of human PI3KCG.
NCBI Gene ID #:
5294
NCBI Official Name:
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
NCBI Official Symbol:
PIK3CG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
126 kDa
Protein Accession #:
P48736
Protein GI Number:
92090623
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Immunology,Signal Transduction
Swissprot #:
P48736
User NOte:
Optimal dilutions for each application to be determined by the researcher.