IGJ Antibody

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ProSci
Product Code: PSI-62-317
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-317-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of IGJ antibody in mouse spleen lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded mouse spleen reacted with IGJ Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of IGJ antibody in mouse spleen lysates (35ug/lane)
Formalin-fixed and paraffin-embedded mouse spleen reacted with IGJ Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Immunoglobulin J chain, IGJ, IGCJ
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
IGJ serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component.
Background References:
  • Ramachandran,P., J. Proteome Res.(6), 1493-1503 (2006)
  • Liu,T., J. Proteome Res.(6), 2070-2080 (2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This IGJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human IGJ.
NCBI Gene ID #:
3512
NCBI Official Name:
Immunoglobulin J chain
NCBI Official Symbol:
IGJ
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
18 kDa
Protein Accession #:
P01591
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Immunology,Signal Transduction
Swissprot #:
P01591
User NOte:
Optimal dilutions for each application to be determined by the researcher.