IL13 Antibody

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ProSci
Product Code: PSI-57-235
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-235-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
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Confocal immunofluorescent analysis of IL13 Antibody with NCI-H460 cell line followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of IL13 Antibody with NCI-H460 cell line followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Interleukin-13, IL-13, IL13, NC30
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50
Background:
This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq].
Background References:
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Shi, X., et al. Anat Rec (Hoboken) 293(9):1470-1476(2010)
  • Beckers, M.M., et al. Eur. J. Intern. Med. 21(4):289-292(2010)
  • Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010) In press :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This IL13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-146 amino acids from the C-terminal region of human IL13.
NCBI Gene ID #:
3596
NCBI Official Name:
Interleukin-13
NCBI Official Symbol:
IL13
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
16 kDa
Protein Accession #:
P35225
Protein GI Number:
239938644
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Immunology,Signal Transduction
Swissprot #:
P35225
User NOte:
Optimal dilutions for each application to be determined by the researcher.