PIK3R3 Antibody

Contact us
ProSci
Product Code: PSI-63-351
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-351-400ul400ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Fluorescent image of U251 cells stained with PIK3R3 Antibody . Antibody was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
2 / 3
Western blot analysis of lysate from MDA-MB-453 cell line, using PI3KR3 Antibody (G331) RB1719 was diluted at 1:1000 at each lane.
3 / 3
❮❯

Fluorescent image of U251 cells stained with PIK3R3 Antibody . Antibody was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Western blot analysis of lysate from MDA-MB-453 cell line, using PI3KR3 Antibody (G331) RB1719 was diluted at 1:1000 at each lane.

Documents

Further Information

Additional Names:
Phosphatidylinositol 3-kinase regulatory subunit gamma, PI3-kinase regulatory subunit gamma, PI3K regulatory subunit gamma, PtdIns-3-kinase regulatory subunit gamma, Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma, PI3-kinase subunit p55-gamma, PtdIns-3-kinase regulatory subunit p55-gamma, p55PIK, PIK3R3
Application Note:
For IF starting dilution is: 1:100

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
PIK3R3 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The protein is expressed at highest levels in brain and testis. Lower levels are detected in adipose tissue, kidney, heart, lung and skeletal muscle. The protein contains 2 SH2 domains.
Background References:
  • Dey, B.R., et al., Gene 209 (1-2), 175-183 (1998).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PIK3R3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-346 amino acids from the C-terminal region of human PIK3R3.
NCBI Gene ID #:
8503
NCBI Official Name:
Phosphatidylinositol 3-kinase regulatory subunit gamma
NCBI Official Symbol:
PIK3R3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
Q92569
Protein GI Number:
317373310
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Immunology,Signal Transduction
Swissprot #:
Q92569
User NOte:
Optimal dilutions for each application to be determined by the researcher.