SERPINF1 Antibody

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ProSci
Product Code: PSI-62-817
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-817-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in human blood plasma tissue lysates (35ug/lane).This demonstrates the SERPINF1 antibody detected the SERPINF1 protein (arrow).
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SERPINF1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis in human blood plasma tissue lysates (35ug/lane).This demonstrates the SERPINF1 antibody detected the SERPINF1 protein (arrow).
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SERPINF1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Pigment epithelium-derived factor, PEDF, Cell proliferation-inducing gene 35 protein, EPC-1, Serpin F1, SERPINF1, PEDF
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
SERPINF1 is a member of the serpin family. Serpins are a group of serine protease inhibitors, some of which have also been reported to exhibit neurotrophic activity.
Background References:
  • Bessho,H., Kondo,N. Mol. Vis. 15, 1107-1114 (2009)
  • Petersen,S.V., Valnickova,Z. Biochem. J. 374 (PT 1), 199-206 (2003)
  • Tombran-Tink,J., Pawar,H. Genomics 19 (2), 266-272 (1994)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This SERPINF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 279-306 amino acids from the Central region of human SERPINF1.
NCBI Gene ID #:
5176
NCBI Official Name:
Pigment epithelium-derived factor
NCBI Official Symbol:
SERPINF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46 kDa
Protein Accession #:
P36955
Protein GI Number:
313104314
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Neuroscience
Swissprot #:
P36955
User NOte:
Optimal dilutions for each application to be determined by the researcher.