CRYAA Antibody
| Code | Size | Price |
|---|
| PSI-60-696-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Alpha-crystallin A chain, Heat shock protein beta-4, HspB4, Alpha-crystallin A(1-172), Alpha-crystallin A(1-168), Alpha-crystallin A(1-162), CRYAA, CRYA1, HSPB4
Application Note:
For WB starting dilution is: 1:1000
Background:
Crystallins are separated into two classes:
taxon-specific, or enzyme, and ubiquitous. The latter class
constitutes the major proteins of vertebrate eye lens and maintains
the transparency and refractive index of the lens. Since lens
central fiber cells lose their nuclei during development, these
crystallins are made and then retained throughout life, making them
extremely stable proteins. Mammalian lens crystallins are divided
into alpha, beta, and gamma families; beta and gamma crystallins
are also considered as a superfamily. Alpha and beta families are
further divided into acidic and basic groups. Seven protein regions
exist in crystallins: four homologous motifs, a connecting peptide,
and N- and C-terminal extensions. Alpha crystallins are composed of
two gene products: alpha-A and alpha-B, for acidic and basic,
respectively. Alpha crystallins can be induced by heat shock and
are members of the small heat shock protein (sHSP also known as the
HSP20) family. They act as molecular chaperones although they do
not renature proteins and release them in the fashion of a true
chaperone; instead they hold them in large soluble aggregates.
Post-translational modifications decrease the ability to chaperone.
These heterogeneous aggregates consist of 30-40 subunits; the
alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two
additional functions of alpha crystallins are an autokinase
activity and participation in the intracellular architecture.
Alpha-A and alpha-B gene products are differentially expressed;
alpha-A is preferentially restricted to the lens and alpha-B is
expressed widely in many tissues and organs. Defects in this gene
cause autosomal dominant congenital cataract (ADCC). [provided by
RefSeq].
Background References:
- Deng, M., et al. Biochim. Biophys. Acta 1802 (7-8), 621-631 (2010) :
- Li, F.F., et al. Curr. Eye Res. 35(6):492-498(2010)
- Pang, M., et al. Biochim. Biophys. Acta 1804(4):948-956(2010)
- Bhagyalaxmi, S.G., et al. OmanOphthalmol 3(1):7-12(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rabbit, Rat
Immunogen:
This CRYAA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the Central region of human CRYAA.
NCBI Gene ID #:
102724652, 1409
NCBI Official Name:
Alpha-crystallin A chain
NCBI Official Symbol:
CRYAA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
20 kDa
Protein Accession #:
P02489
Protein GI Number:
1706112
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Neuroscience,Signal Transduction
Swissprot #:
P02489
User NOte:
Optimal dilutions for each application to be determined by the researcher.