LIMK2 Antibody

ProSci
Product Code: PSI-60-969
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-60-969-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Immunohistochemical analysis of paraffin-embedded H.kidney section using LIMK2 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
2 / 2
Western blot analysis of lysates from Jurkat cell line and human placenta tissue lysate (from left to right), using LIMK2 Antibody at 1:1000 at each lane.

Immunohistochemical analysis of paraffin-embedded H.kidney section using LIMK2 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from Jurkat cell line and human placenta tissue lysate (from left to right), using LIMK2 Antibody at 1:1000 at each lane.

Further Information

Additional Names:
LIM domain kinase 2, LIMK-2, LIMK2
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This LIMK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 570-599 amino acids from the C-terminal region of human LIMK2.
NCBI Gene ID #:
3985
NCBI Official Name:
LIM domain kinase 2
NCBI Official Symbol:
LIMK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
72 kDa
Protein Accession #:
P53671
Protein GI Number:
1708824
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Neuroscience,Signal Transduction
Swissprot #:
P53671
User NOte:
Optimal dilutions for each application to be determined by the researcher.